(4pts) After you performed the freeze-thaw cycle to lyse the bacteria, you pelleted the debris using a centrifuge. What two reasons did this pellet fluoresce green? (Yes, I know it contained GFP…. but why?)

one is performing the freeze-thaw cycle to lyse the bacteria and pelleted the debris using a centrifuge then noticed that the pellet was fluoresce green

there can be some possibilities, such as-

the GFP must have been covered up by the debris, that must have made the pellet fluoresce.there must be some non-lysed cells in the pellet as the thawing must have not been done efficiently.this inefficient freeze-thawing can make GFP to be pelleted down with cell debris rather than in the supernatant. because of all these E.coli would still contain some GFP protein.

B. absorbs violet lightto get absorb sunlight for photosyntesis