It can be desirable to produce eukaryotic proteins in prokaryotes such as E. coli. To do this several DNA sequences must be joined or cloned together. Place the labels in the appropriate position to allow for the expression of the insulin protein in a prokaryotic cell. cDNA is DNA that is synthesized from the mature mRNA from eukaryotic cells using the enzyme reverse transcriptase.

5' end 3' end

1. Poly A signal sequence
2. Genomic clone of insulin
3. Rho terminator
4. -95 CAT box
5. -10 TATA
6. -25 TATA box
7. cDNA of insulin
8. -35 Sequence