Acid-fast organisms such as Mycobacterium tuberculosis resist decolorization by an acid-alcohol wash because of the high concentration of in their cell walls.

Acid -fast organisms resist decolonization by an acid alcohol wash because of high concentration of lipid in their cell wall.

Explanation:

Acid fast organisms contain a wax like impermeable cell wall which contain mycolic acid and large amount of fatty acid,waxes and complex lipid.Acid fast organisms are highly resistant to disinfectants and dry condition Mycobacteria possess thick waxy cell wall rich in mycolic acid. This makes it difficult to be stained using Gram's techniques. But with the use of carbon fuschen,the cell wall can be stained using acid-fast technique.

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.

light dependent reactions:

light independent reactions:

hope this !