Protein X is a protein that is composed of four subunits of equal size--alpha, beta, delta, and gamma--that are bound by either noncovalent interactions or disulfide bridges. Only the beta and delta subunits are bound to each other by disulfide bridges. How many bands would you expect to see in an SDS PAGE experiment in which beta-mercaptoethanol, a reducing agent, is used?

One.

Explanation:

SDS (sodium dodecyl sulfate) PAGE ( Polyacrylamide gel electrophoresis) is the technique of the separation of the molecules on the basis of their molecular weight.

The beta-mercaptoethanol reduces and break the disulfide bonds present in the protein. When the protein X with the beta-mercaptoethanol is allowed to run on the gel, only one band will visible all the subunits have equal weight and will present at the same location on the gel.

Thus, the answer is one.

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-lavira: )

an individual’s sex (i.e., whether they are a male or female) is determined by the sex chromosomes. most people have two sex chromosomes, one that is inherited from their mother and one that is inherited from their father. typically, females have two x chromosomes (xx) and males have one x chromosome and one y chromosome (xy). conditions caused by changes (“mutations”) in genes located on the x chromosome are considered x-linked.

x-linked recessive inheritance

most x-linked conditions are recessive. this means that in a person with two x chromosomes (most females), both copies of a gene (i.e., one on each x chromosome) must have a change or mutation whereas in a person with one x chromosome (most males), only one copy of a gene must have a mutation. a female with a mutation in one copy of a gene on the x chromosome is said to be a “carrier” for an x-linked condition. a male with a mutation in a gene on the x chromosome is typically affected with the condition. because females have two copies of the x chromosome and males have only one x chromosome, x-linked recessive diseases are more common among males than females. however, x-linked recessive diseases can occur in both males and females.

for x-linked recessive disorders, an unaffected carrier mother who has a mutation in a gene on the x chromosome can transmit either the x chromosome with this mutation or a “normal” x chromosome to her children. if the father is unaffected, none of her daughters will be affected and all of her daughters will be unaffected—since they will inherit at least one normal x chromosome from their father. however, each daughter will have a 50% chance of being an unaffected carrier like her mother and a 50% chance of both x chromosomes being normal.