subject
Biology, 27.02.2020 17:59 jxeidy8284

Allele specific oligonucleotides (ASO) can be used to determine the different alleles present in a DNA sample. Single sperm, which have completed meiosis, can be used for a polymerase chain reaction (PCR). The amplified DNA is then spotted to filters and hybridized with probes that recognize single nucleotide differences. A sperm sample was collected from a single individual and DNA was extracted from individual sperm. For each DNA sample, four different regions of the genome were amplified using PCR (called A, B, D, and E). For each DNA sample, ASO probes were used to determine the allele present (Either allele 1 or 2) for each region of the genome amplified. X indicates the allele is present and - indicates the allele is not present. Number of Sperm Loci and AllelesA1 A2 B1 B2 D1 D2 E1 E245 X - X - X - - X46 - X X - - X X -7 X - X - X - X -8 - X X - - X - X17 X - X - - X - X19 - X X - X - X -(Notice that there are only six classes of genotypes among the products of meiosis. For three segregating genes, you would expect to see eight different genotypic combinations. The missing genotypes are the DCO combinations and thus you should figure out what combinations are missing and assign them a value of zero)What is an estimate of the map distance for each pair of genes?

ansver
Answers: 1

Another question on Biology

question
Biology, 22.06.2019 01:00
The picture shows a fishing technique called trawling. how might trawling affect marine biodiversity
Answers: 1
question
Biology, 22.06.2019 07:00
Give two examples of what would occur if one of the pairs did not match or had an extra chromosomes. explain how this might occur, and if it would be dangerous to the individual. fast.
Answers: 1
question
Biology, 22.06.2019 08:00
Which best example best demonstrates the importance of having knowledge of evolutionary relationships? a. illustration of a plant b.organ transplantation between species c. blood donation from a human d. do you need sequence of an insect.
Answers: 1
question
Biology, 22.06.2019 11:30
_tissues respond quickly to outside stimuli a) epithelia b)nervous c)muscular d)muscular and nervous
Answers: 2
You know the right answer?
Allele specific oligonucleotides (ASO) can be used to determine the different alleles present in a D...
Questions
question
Arts, 04.12.2020 19:20
question
Mathematics, 04.12.2020 19:20
question
Chemistry, 04.12.2020 19:20
question
Mathematics, 04.12.2020 19:20
question
Biology, 04.12.2020 19:20
question
Engineering, 04.12.2020 19:20
question
Mathematics, 04.12.2020 19:20
question
Biology, 04.12.2020 19:20
question
Mathematics, 04.12.2020 19:20
question
Mathematics, 04.12.2020 19:20
Questions on the website: 13722362