A biologist wants ipecifically to examine the surfaces of different types of cells in kidney tubules be distinguished by external shape, size, and 3-D The cells in question can of small mammals characteriatics Which of the following would be the optimum method for her study? A) light microscopy of living unstained material B) cell fractionation C) transmission electron microscopy D) light microscepy using stains specific to kidney furction E) scanning electron microscopy

E) scanning electron microscopy

Explanation:

A biologist who was studying kidney tubules in small mammals wants to specifically examine the juxtaposition of different types of cells in these structures. The cells in question can be distinguished by external shape, size, and 3-dimensional characteristics using a Scanning electron microscopy.

Scanning electron microscopy (SEM) is used to examine the ultrastructural morphology of the cells of the kidney tubules. The Material for study is isolated and fixed by vascular perfusion. SEM is a form of electron microscope that gives pictorial view of a sample using a focused beam of electrons to scan the sample to reveal the surface topography and constituents of the sample.

SEM can produce resolution that is more than 1 nanometer.

Specimens are viewed in high vacuum in conventional SEM, or in low vacuum or wet situations in different pressure or environmental SEM, and at a broad set of cryogenic or increased temperatures with specialized tools.

Advantages of a Scanning Electron Microscope are its broad-array of uses, the well structured three-dimensional and topographical imaging or pictorial representation plus the varying information assembled from seperate detectors.

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