Suppose your professor handed you a test tube with 3 ml of an E. coli culture in it and told you to make a 10-3 dilution of the entire culture volume with water. What would your final volume (V2) of the dilution need to be in order to get this dilution?

Explanation:

The correct dilution that you are asked to make is a 10⁻³ dilution of the entire culture volume with water.

That means a 1,000 fold dilution.

Dilutions of a culture are calculated using the equation:

Where,  and are the volume and concentration of the orginal culture and and  are the volume and concentration of the diluted culture.

         1,000 folds dilution means   or

Hence:

This is, 3ml must be converted into 3,000 ml by adding pure water.

c. would be the answer : )

explanation: