Mental experiment. Picture in your mind the velocity vs. substrate concentration curve for a typical Michaelis-Menten enzyme. Now, imagine that the experimental conditions are altered as described below. For each of the conditions described, fill in the table indicating precisely (when possible) the effect on Vmax and KM of the imagined Michaelis- Menten enzyme.

Experimental condition Vmax KM

a. Twice as much enzyme is used.
b. Half as much enzyme is used.
c. A competitive inhibitor is present.
d. An uncompetitive inhibitor is present.
e. A pure noncompetitive inhibitor is present.