The introductory passage described a consortium consisting of ANME and a sulfur oxidizing bacteria. Which of the following would be important considerations in choosing the best technique to determine the number of living cells in a culture of the consortium?

Select the best answer.

A) Some fluorescent stains react differently based upon whether a cell has an intact cell membrane.
B) Phylogenetic stains can distinguish between species of Bacteria and species of Archaea.
C) DAPI can be used to stain DNA.
D) Cells present in low concentrations can be counted using microscopic cell counts, but they must be concentrated first.
E) Small cells are more difficult to see when doing microscopic cell counts.

C) DAPI can be used to stain DNA.

Explanation:

DAPI is a technique used for the effective quantification of bacteria by staining. This technique allows to visualize the DNA in living and fixed cells. It is a fluorescence technique, after this, quantify with the help of the electron microscope.

then that particular organisms become extinct.

correct answer is option a.

tropopause, stratopause and mesopause have all in common aspect that is point of maximum temperature in its layer of the atmosphere. all layers of atmosphere observe abrupt change in temperature. tropopause is the boundary between troposphere and stratosphere. stratopause is the boundary between stratosphere and mesosphere. mesopause is the boundary between mesosphere and thermosphere. these all boundaries are ranges between altitude of 0 to 80 km.