In the shotgun approach to whole-genome sequencing (shotgun sequencing), random dna fragments of a chromosome are sequenced. the fragment sequences are then assembled into a continuous sequence that represents the dna of the entire chromosome.

Remainder of question from another source:

What are the steps in the shotgun approach to whole-genome sequencing?

PLACE IN ORDER

A. 1-kb fragments are cloned into plasmids.

B. Chromosomes copies are broken into 1-kb fragments

C. The plasmids are sequenced.

D. Multiple copies of the same chromosome are prepared.

E. A computer combines the fragments sequences.

DBACE

Explanation:

First, multiple copies of an entire chromosome are created (D). Then, the chromosome is fragmented into lots of pieces approximately 1kb in length. These pieces will contain overlapping sequences (B). Next, the newly created 1kb fragments will be cloned into plasmid vectors (A).  The plasmids containing the DNA fragments are then sequenced (C), and the sequence is combined and aligned by a computer, aided by the fact the fragments are overlapping (E).

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.

a- multicellular

b-eukaryotic

c-no cell wall

d-heterotrophic

e-autotrophic