If you concluded that gene x is necessary, describe an experiment to test whether gene x is sufficient. if you concluded that gene x is sufficient, describe an experiment to test whether gene x is necessary.

Knock down the gene to see it is sufficient

Knock out the gene see if it is necessary

Explanation:

The expression of the Gene X can be changed by inserting a promoter which can control the expression like lacZ. Then with this promoter effect you can see what happens when the expression of Gene X is lower.

To see its necessity, the gene can be knock down by CRISPR Cas9 or other techniques. You can see the effect when it is not translated at all.

primary succession begins with a bare rock and ends with a forest secondary succession begins after a natural disaster where there is a temporary setback after a forest fire or local destruction, but the surrounding area can fill in