You identify an rflp in mice by digesting genomic dna with hindiii enzyme and radiolabeling a piece of probe dna. southern blot analysis shows that the probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice. the two mice strains are crossed to produce an f1 generation. two f1siblings are mated to produce a dozen f2 progeny. you isolate genomic dna from several f1 and f2 individuals, digest with hindiii, and perform a southern blot with the same probe. how many bands would be seen in the southern blot of an f1 individual? a. twob. fourc. one or twod. onee. three

Part 1 =          (A) Two

Part 2 =         (B) Cleavage of X-gal

Explanation:

Part 1: As   probe detects a 2-Kb fragment in one strain of mice and a 4-kb fragment in another strain of mice.Thus 2 bands would be seen in the Southern blot of an F1 individual.

Part 2: The antibiotic & Xgal markers provide a selection scheme for recombinant bacterial colonies, as follows. First, only bacterial colonies that contain a plasmid-borne antibiotic-resistance gene can grow at all. Second, of those that grow, only those whose plasmids carry a functional LacZ in the poly-linker can metabolize Xgal.

b would be the right answer