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Biology, 19.12.2019 00:31 lilay30

X-gal is a synthetic molecule which is a substrate for beta-galactosidase (b-gal). when cells are grown on a medium containing x-gal, they turn blue if the cells are expressing b-gal. in the absence of b-gal expression, the cells are white. you are attempting to clone a gene of interest into a lacz reporter gene of a plasmid and introduce your recombinant molecule into a bacterial host sensitive (i. e. not resistant) to ampicillin. the plasmid contains a gene for ampicillin resistance at a position other than the cloning site. you grow your potential transformants on media containing both ampicillin and x-gal. which of the following results would most likely indicate a successful clone?

cells which form blue colonies on the ampicillin plates
cells which form white colonies on the ampicillin plates
cells which form light blue (half blue, half white) colonies on the ampicillin plates
cells which do not grow on the ampicillin plates
none of the above results would indicate a successful clone.

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