Targeted gene knockouts involve the deletion or disruption of a given gene. what method might be effectively used to knock out a gene in yeast? what method might be used in mice? why would the method used in yeast be ineffective in mice?

In order to determine the function of an unknown gene knockout technique is used. PCR based deletion strategy is used for gene knockout in the case of yeast, which is unicellular species and eukaryotic. On the other hand, in the case of a mouse, which is multicellular and eukaryotic, for gene knock out homologous recombination technique is used.  

The PCR based strategy is not appropriate for mice genes knockout, as mice are multicellular species and exhibit complex genome. While yeast constitutes some circular plasmids, known as 2 microns plasmids, which allows PCR based mutagenesis.  

mountains can affect the climate of nearby lands. in some areas, mountains block rain, so that one side of a mountain range may be rainy and the other side may be a desert. much of airborne moisture falls as rain on the windward side of mountains.

explanation:

i think a) betsy can donate only blood to mark is correct because, mark and betsy have the same blood type. whereas nobody else has b+ blood so betsy can't donate to them.