In general, what length of oligonucleotide would be sufficient to amplify a single unique sequence? to simplify your calculation, assume that all bases occur with an equal frequency. hint: the frequency of occurrence of a pcr primer in genomic dna is given by this equation: genomic dna length × sequence frequency the sequency frequency is (1/4) n , where n corresponds to the number of nucleotides in the site. express your answer as an integer.

estuaries are the partially enclosed coastal bodies of brackish water formed at the mouth of sea where river meets an ocean. it is a transition zone between river and sea water so estuaries are influenced to both marine environment such as tides, waves and to riverine such as flow of fresh water and sediments. the mixing of sea and fresh water provide high level of nutrients, making estuaries a high product habitats.

many estuaries suffers degradation from a variety of human activities like erosion from deforestation, overgrazing, overfishing, drainage and eutrophication.

so from the above statement answers will be

1. false as estuaries are formed due to both brackish and freshwater.

2.true, due to high productivity estuaries are breeding ground for many marine species.

3. direct and indirect activities of man that contribute to pollution and deposition of sediments.

answer >   yes we do, women; have three holes, urethral opening(pee hole, vagina, and the women pee/girls pee out of the urethra like the men do; only they have a single opening to pee/ejaculate.   (sorry if i'm too forward with this, but this is for a biology class, isn't it? )

hope that !         : )