For this assignment, you are a member of a research group interested in determining the effect of tryptophan (an amino acid) on the expression of five genes in e. coli (listed in the table below). in your first experiment you are going to compare gene expression for the organism grown in minimal medium in the absence or presence of tryptophan. the table below describes the five genes we will examine. the function of each gene is listed beside its name. gene name description ryptophan-specific transport protein-transports tryploghan inte the cell tryptophan synthase, a protein- synthesizes tryptophan from its basic components periplasmic copper binding peotein binds copper ions tryptophanase- enryme for tryptophan catabolism tryptophan synthase,ロprotem-syntheses trypsoplan trpa cusf pb you purchase a microarray composed of the above genes from a biotechnology company. the microarray that you select contains spots of single stranded probes for each of the 5 genes. e. coli microarray you then proceed to do the following experiment: you grow a wild type e. coli culture in minimal medium (min) and in min containing an excess of l-tryptophan (50 ug/ml) (min +trp) cells are removed from each culture, and mrna is prepared. the cdna from the min culture is labeled with a red fluorophore and the cdna from the min trp culture was labeled with a green fluorophore. the cdnas are hybridized onto the microarray, and the data collected is below )graph the patterns of gene expression for each of the 5 genes. 2) answer the following questions on your own. type vour answers and submit. a.) how did the level of gene expression change (increase, decrease, stay the same) when the cells were supplied with tryptophan in the medium (min+trp) medium (min)? was red, red-yellow, yellow, yellow-green, or green. explain compared to minimal b.) for each of the 5 genes, state whether its spot on the microarray briefly c.) if tryptophan caused the expression level to change, explain why these changes occur (that is, how does the presence of tryptophan in the media influence expression of the based on each gene's known function). should be able to infer this d.) if no change in expression was observed, explain why the