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Invertase is a yeast enzyme that enters the secretory pathway and is targeted to the fungal vacuole, an organelle important for the storage and digestion of small molecules. put3 is a transcription factor that constitutively occupies enhancers for proline-sensitive genes. for some reason you splice a gfp domain with a c-terminal kdel sequence to the genes for both invertase and put3 and express these gene constructs in two different s. cerevisiae lines. predict where you will see fluorescence in cells of each engineered line and explain your predictions.
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Invertase is a yeast enzyme that enters the secretory pathway and is targeted to the fungal vacuole,...
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