You are tasked with making a 40 nucleotide dna sequence via solid phase techniques from phosphoramidite building blocks. you know that your goal is to generate a minimum of 0.5 mmol of the final product. your individual coupling efficiencies are 96% which gives you a 20% overall yield. what is the minimum amount of the 3’ terminal building block you should start with to meet your goal?

step 1: replication fork formation.

step 2: primer binding.

step 3: elongation.

step 4: termination.

explanation:

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what's the question?

the nitrogen cycle provides nitrogen to the ecosystem from the atmosphere, ground and oceans. nitrogen is an important component of complex molecules such as amino acids and nucleotides, which lead to the creation of proteins and dna, the building blocks of all life. plants absorb nitrogen in the form of ammonium, nitrate ion and, on rare occasions, as amino acids. animals receive nitrogen necessary for biological processes from feeding on living or dead organic matter.

1) a

2)c

3)a

4)c

5)b

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