Proteins often bind to dna to affect it's function. where do most proteins bind to on the dna?
a. minor groove
b. telomere at end of dna
c. major groove
d. sugar phosphate backbone

c. Major groove

Explanation:

The interaction surfaces of proteins are, in general, varied. From the point of view of the secondary structure, the surfaces of the proteins are formed by the surfaces of their α helices, β sheets and loops that conform them. The surfaces of the DNA molecules are less varied: the B-DNA helix has a monotonous “screw” shaped surface with phosphoribose ridges between which two grooves (major and minor) are formed. The difference between different nucleotide sequences can only be seen from outside in the major groove, where the bases appear. The surfaces of RNA molecules that possess tertiary structure (such as t-RNA) are almost as complex as that of proteins.

Since the major groove is the only site where the bases are accessible from outside the B-DNA without distorting it, the major groove constitutes the main recognition site. As the interaction between molecules is stronger if their surfaces are complementary, the protein-DNA interaction usually occurs by filling the major groove.

ans.

dna fingerprinting is defined as a process that determines the dna characteristics of an individual. these dna characteristics are unique among individuals as their fingerprints, so it is known as dna fingerprinting.

steps of dna fingerprinting includes extraction of dna from sample, amplification of dna, restriction digestion of amplified dna, and then analysis of dna by gel electrophoresis, respectively.

thus, the next step performed by the scientist is 'to cut the amplified dna (obtained by polymerase chain reaction) by restriction enzymes that cleave the dna into fragments and their analysis.'