Biology, 10.10.2019 21:10 Nigward666
Suppose you are working on a set of experiments using western blotting. using an antibody that recognizes amino sequences encoded by exon 3 of a five-exon gene, you have demonstrated that a protein is highly expressed in a particular cell line. to assess the mrna, you design reverse transcriptase polymerase chain reaction (pcr) primers to exons 2 and 4, which flank exon 3. but despite all your efforts, you never detect an mrna. you know that it is indeed expressed, since the protein is detectable. you also know that your "positive controls" are working and your experimental procedure is sound, since the use of other cell lines allows you to readily detect an mrna. given what you know about the "central dogma" and gene structure, how might you explain the inability to identify the mrna encoding this protein?
Answers: 1
Biology, 21.06.2019 19:50
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Biology, 22.06.2019 07:30
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Biology, 22.06.2019 13:30
Where is most of the fresh water on earth found a in the ocean b in glaciers and in ice caps c in rivers d in the soil
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Suppose you are working on a set of experiments using western blotting. using an antibody that recog...
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