Biology, 20.09.2019 00:20 merber00

You experimentally change the dna sequence directly upstream of a start codon of an operon in e coli to investigate the function of this region of dna. analysis reveals that after the change the same amount of mrna is made from the operon, but there are very few proteins made from the operon. what is the most likely function of the dna sequence that you changed?

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Mutation occurred in ribosome binding site that serves as binding site for 30S ribosomal subunit of E. coli and allows the process of protein synthesis to begin.

Explanation:

The initiation of protein synthesis in bacteria requires binding of the ribosome to the ribosome binding site. The ribosome binding site in bacteria consists of the initiation codon "AUG" and the preceding Shine-Dalgarno sequence. The AUG initiation codon and Shine Delgrano sequence are around 10 bases apart.

The sequence is polypurine hexamer and is represented by 5' ...AGGAGG...3'. Shine-Delgrano sequence is complementary to the conserved sequence present at the 3' end of 16SrRNA of the 30S subunit of the bacterial ribosome. Binding of Shine Delgrano sequence of ribosome binding site and the complementary sequence of the 30S ribosomal subunit marks the first step in the initiation of protein synthesis.

Any mutation in the ribosome binding site would not allow the process of protein synthesis to start or would reduce the rate of the initiation of protein synthesis.

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It allows water to resist freezing and thawing cycle.

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there are mainly three domains in biological classification namely; archaea, bacteria, and eukarya.

archaea includes all the prokaryotic cells which are characterized by their lipid membranes. they have branched hydrocarbon which is attached to glycerol through an ether linkage.

bacteria includes all the prokaryotic cells, the cell membrane of which are made up of the unbranched fatty acid chain. in addition, they are attached to glycerol through an ester bond.

eukarya includes all the eukaryotic cells, that is, the cells contain a nucleus and other membrane-bound cell organelles.

they can be autotrophic as well as heterotrophic, unicellular as well as multicellular

they include kingdoms like protista, plantae, animalia, fungi et cetera.

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You experimentally change the dna sequence directly upstream of a start codon of an operon in e coli...

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