Why must reverse transcriptase be used to create a eukaryotic expression library?
a. reverse transcriptase is only used to create prokaryotic expression libraries
b. reverse transcriptase creates cdna from mrna in prokaryotes
c. reverse transcriptase ensures the gene is in the correct orientation within the expression vector to create protein
d. reverse transcriptase creates cdna from mrna because genes in eukaryotes have large numbers of non-coding regions.
e. no other enzymes are used to create expression libraries except restriction enzymes

Option (d).

Explanation:

Genomic library may be defined as the total genome DNA of the organism. Reverse transcriptase is the enzyme used for the formation of DNA from the RNA molecule.

The eukaryotes DNA contain large number of sequences called introns that creates a problem in the construction of the eukaryote genomic library. This problem can be solved by creating the complementary DNA from the RNA molecule free from the introns.

Thus, the correct answer is option (d).

blue stars have the highest surface temp

stellar distances are usually expressed in light-years

the measure of a star's brightness is called magnitude

hope this !

water is a polar molecule. a chemical bond is a force of attraction between atoms or ions. bonds form when atoms share or transfer valence electrons. atoms form chemical bonds to achieve a full outer energy level, which is the most stable arrangement of electrons. so, false

explanation:

in h2o molecule, two water molecules are bonded by a hydrogen bond but the bond between two h - o bonds within a water molecule are covalent. the greater the electronegativity difference, the more ionic the bond is. bonds that are partly ionic are called polar covalent bonds. nonpolar covalent bonds, with equal sharing of the bond electrons, arise when the electronegativities of the two atoms are equal.