Which of the following are useful traits of cloning vectors?
a. an antibitioc resistance gene on the plasmid for selection of cells containing the plasmid
b. a site that contains unique, clustered restriction enzyme sequences for cloning foreign dna
c. a high copy number plasmid so that large amounts of dna can be obtained
d. alpha complementation to determine if the foreign dna was inserted into the cloning site
e. all of the above are useful traits

The correct answer is option - e. all of the above are useful traits.

Explanation:

A cloning vector is a small segment of DNA that can be established stably in an individual organism and able to internalize a foreign DNA for cloning purposes. The cloning vector could be taken from different organisms such as plasmid of a bacterium, or DNA from a virus. A cloning vector must possess some characteristics -  

The cloning vector must be self-replicating ability in the host cells, it should be small in size with restriction site for the restriction endonuclease enzymes in it. The replication character of the vector should not interfere with the internalized DNA fragment. The presence of marker genes in the vector is also a property that is helpful in identifying of recombinant cells.

Thus, the correct answer is option - e. all of the above are useful traits.

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