Below are steps performed to make an e. coli bacteria that can make human insulin protein for the health care market. list these steps in their proper order.
a. use heat shock or electroporation to get the recombinant human insulin gene to go into a living e. coli cell.
b. grow the e. coli in fermentation culture, extract and sell humulin.
c. modify the human gene and give it a promoter that will be turned on in e. coli cells.
d. extract the dna from a human who does not have diabetes
e. isolate and clone (make copies of) the gene encoding the insulin protein

The following are the steps for making the human insulin protein for the health care market:  

1. Obtain the DNA from a human being who does not have diabetes.  

2. Modify the human gene, and provide it a promoter, which will get turn on when placed within the E.coli cell.  

3. Separate and make copies of the gene encrypting the insulin protein.  

4. Application of electroporation or heat shock method in order to administer the human insulin gene within the living E.coli cell.  

5. Development and growth of E.coli in the fermentation culture, and then extract and sell the humulin in the market.  

"d.) carbon dioxide and hydrogen sulfide"

answer: decrease

a limiting factor is the one which decrease the abundance, distribution and causes extinction of the population of species in a ecosystem. here, predators including hawks, owls and snakes are the limiting factor for the population of mexican free tailed bat.   although bats are nocturnal animals as they forge for food at night these predators also may remain active during night which can decrease the population of the mexican free-tailed bats. also, these bats eat only one type of insects that are moths. the food can also be a limiting factor as all 400 members are dependent upon same type of food needs that are moths. the decrease in the moth population can cause decrease in population size of the mexican free-tailed bats.

therefore, the scenarios will most likely to decrease the population size of the mexican free-tailed bats.