Agenetic engineer needs to use gene therapy to a person with cystic fibrosis. arrange the following steps in the order the engineer would use them.
a. use only the steps you need. inject the modified cfrt gene into a fertilized egg and implant into a woman who will be the surrogate mother.
b. combine the cloned cfrt gene with a disarmed respiratory virus.
c. clone the cfrt gene from someone with cystic fibrosis.
d. clone the cfrt gene from someone without cystic fibrosis.
e. modify the cfrt gene by putting on a different promoter
f. test the patient’s blood cell dna with pcr to see if they have the cfrt transgene.
g. have the patient use an inhaler that contains the modified respiratory virus.

d-b-g-f

Explanation:

1. Clone the CFRT gene from someone without cystic fibrosis.

This will make millions of copies of the gene (wild type, not being mutated and thus unable of producing the disease).

2. Combine the cloned CFRT gene with a disarmed respiratory virus.

This step will allow the virus to transport the gene of interest.

4. Have the patient use an inhaler that contains the modified respiratory virus.

This step helps the virus to enter and infect the patient's cells and thus allowing the copies of the transgene to be integrated into the patient's genome.

3. Test the patient's blood cell DNA with PCR to see if they have the CFRT transgene.

This will confirm if the transgene has actually been integrated into patient's genome.

a researcher conducts an experiment on the secretion of a particular hormone in mice. scientists inject mice with a substance that stimulates the production of the hormone. the scientists then test the levels of hormones produced by the mice. the tool used to measure the hormones consistently detects the levels at 10 points lower than the actual hormone levels in the mice. this tool makes measurements, but the measurements aren't .

explanation:

no u