We next want to calculate the concentration of dna of your pcr product in ng/ μl. what was loaded into your dna, however, was not pure pcr product. you loaded a mix of pcr product and blue juice (=loading dye). of the 10 μl of sample you loaded, how many were pcr product and how many were blue juice? μl pcr product + μl blue juice = 10 μl sample3. to determine the concentration of your pcr product in ng/ μl, use your answers to #1 and the first blank of # ng of dna/ μl pcr product = ng dna/ μl pcr product4. if your 16s rrna band is twice as bright as the 500bp ss, what would be the concentration of your pcr product in ng/ μl?