There are 20 different amino acids. what makes one amino acid different from another?

Amino acids specialize in their aspect of production, for instance one type of Amino Acid could code for a specific trait and another codes for another trait and so on. So, Amino Acids specialize in establishing in coding for one part of a gene.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.