Actin filaments have polarity. this means that the two ends can be identified due to structural differences. the plus end is the end to which subunits are added more rapidly, or the end of polymerization. which of the following would enable you to identify the plus end of actin filaments? determining the ionic charge of the ends of the actin filamentsadding radiolabeled actin subunits to a mixture of actin filaments in which conditions are favorable for polymerizationadding radiolabeled actin subunits to a mixture of actin filaments in which conditions favor depolymerization

adding radiolabeled actin subunits to a mixture of actin filaments in which conditions are favorable for polymerization

Explanation:

The basic unit of actin filaments is G-actin or monomeric, globular form of actin. Actin polymerization is a process in which subunits are linking together in order to form filament. Monomers are rapidly added to the + end. Polymerization process can be divided into three stages:

•the nucleation phase-formation of actin nucleus which is composed of three actin monomers

•the elongation phase-adding of actin monomers to the + end (usually helped by elongation factors such as formin) which must be exposed, capp-free

•steady phase

Favorable in vitro condition that induces polymerization can be achieved by addition of salts to a solution of G-actin.  

ans.

dna fingerprinting is defined as a process that determines the dna characteristics of an individual. these dna characteristics are unique among individuals as their fingerprints, so it is known as dna fingerprinting.

steps of dna fingerprinting includes extraction of dna from sample, amplification of dna, restriction digestion of amplified dna, and then analysis of dna by gel electrophoresis, respectively.

thus, the next step performed by the scientist is 'to cut the amplified dna (obtained by polymerase chain reaction) by restriction enzymes that cleave the dna into fragments and their analysis.'