In the 1950s, christian anfinsen demonstrated the renaturation of the protein ribonuclease (rnase) in vitro. after reduction (to reduce the disulfide links) and the addition of urea (to denature the protein), the protein was in an unfolded state. after removing the urea and the reducing agent, the protein refolded, with greater than 90% activity. if the urea were removed after oxidation occurred, the protein had less than 5% activity. why would the protein not refold correctly if the urea were removed after the reducing agent was removed? (in other words, what would happen if the urea were removed after oxidation? )

Monarch butterflies have brightly colored orange wings with black patterns on them, making them easily visible to birds that eat butterflies and moths, yet birds rarely eat the monarchs. likewise, the monarch caterpillars are brightly striped yellow and white and black, and they also are rarely eaten by birds, although some wasps will attack them and feed them to their young. what can be inferred from these observations? monarchs must threaten and attack birds, but not wasps. monarchs have a way of discouraging bird predation that does not involve hiding. monarchs’ wings are so colorful that most birds must find them difficult to eat. monarchs lay more eggs than other less conspicuous butterflies.