Amixture of the amino acids leucine, glutamate, and arginine, is added to a cation exchange column at neutral ph. in what order will they elute from a cation exchange column

Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3,  ~6  ~10.

relationships aren't everything. people everyday spend there entire life depending on reltionships and for someone to be there for them when this is absurd. why must people depend on someone else to bring them happiness? i'm not saying it wrong to feel happy with someone, but people feel that they need to be in a relationship in order to feel happy, when, this is not true. you can paint, write, read ir slend tume with family ir even spend time working in yourself. this can be an exciting experience to find yourself. it's bot wrong to be in a relationship but if you let it be your only source of happiness, than you will never find true happiness.

so, relationships are not always worth it. if you are so dependent on them, than in the end they arent worth it because you will realize how unhappy you really were. some people even tend to cut ties with family because they think they cabt make them happy, obly realtionships with a lover can. false. in order to find true happiness, one must learn to love themselves if they want to love someone else. it's unhealthy to be always depend on relationships and to alqays be in and out of one.

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segmentation=endoskeleton=backbone=coelom=tissues