List the steps of the gram stain technique and describe the purpose of each. be sure to include a description of the color of the bacteria during each step.

The procure of gram staining is as follows:

1. Prepare the smear on a microscope slide and heat-fix it

2.Flood the smear with crystal violet on a staining tray

3.Gently and lightly rinse the smear with sterile water from a wash bottle

4.Flood the smear with iodine for a minute

5.Rinse the smear like in step 3

6.Pour 95% ethyl alcohol or acetone to decolorize for 10 seconds

7.Rinse like in step 3

8.Flood the smear with safranin (a counter-stain) for about 1 minute

9.Rinse the smear like in step 3

10.Leave the smear to air dry and observe under a microscope

Heat-fixing adheres the smear to the slide and hence makes it less amenable to being washed away during gram-staining procedure. Gram staining using crystal violet allows the peptidoglycan layer of the Gram + bacteria to pick up the dye and retain it even after decolorizing. Gram- bacteria lose the crystal violet color after decolorizing with alcohol since they lack the peptidoglycan layer. Safranin is a counter stain since it is picked by the Gram – bacteria that have lost the crystal violet dye. Therefore under a microscope and after the staining procedure, Gram+ bacteria appear violet/purple in colr while Gram – appear pink.

answer: angiosperms

dicots

angiosperms are highly evolved plants, they produce seeds which are enclosed within in a carple. these are the largest group of plants on earth. they include trees, shrubs, herbs and small flowering plants. angiosperms exhibit a flowering stage in their life cycle. the flower serves the function of reproduction and fruit formation in majority of angiosperms. dicot is the term used to describe flowering plants that have two seed leaves. dicots are plants which develop seeds which split into two parts called cotyledons. each cotyledon contains one embryo.

here, a small purple flower development indicates that the plant belongs to angiosperms and having two embryonic leaves indicates it belongs to dicots.