Why is it essential that the same restriction enzyme be used to cleave (cut) the dna of both organisms used to create a transgenic organism?

What mistake did john needham make that caused him to conclude that spontaneous generation for microorganisms occurred? a. he re-contaminated his boiled broth solutions. b. he destroyed the vital force in the solutions. c. he did not boil his broth solutions, only warmed them. d. he failed to seal his flasks of boiled broth. e. he allowed his assistant to conduct the experiment which he did not monitor closely.

Collin would like to use the data a coworker has entered in a database. the problem is the coworker's database software is different from collin's. collin should

I've created a synthetic cellular membrane that is made up of phospholipids, but contains no proteins. which of the types of movements across a membrane would not function with my synthetic membrane?