Advanced Placement (AP), 28.02.2020 07:27 ashiteru123

EP, a 26-year-old Caucasian female student, tested her own blood during a student lab on antibody identification. She had two uneventful prior pregnancies and deliveries. She was told that she had a red cell antibody of “undetermined specificity.” A panel tested with LISS demonstrated the following reactions.

Rh MNSs P1 Lewis Lutheran Kell Duffy Kidd LISS
Cell D C E c e f CW M N S s P1 Lea Leb Lua Lub K k Fya Fyb Jka Jkb is 37 AHG
1 R1R1 + + 0 0 + 0 0 + + + 0 + 0 + 0 + + + 0 + + 0 0
0
2+
2 R1R1 + + 0 0 + 0 + + 0 0 + + 0 + 0 + 0 + 0 + + + 0 0 2+
3 R2R2 + 0 + + 0 0 0 0 + 0 + + + 0 0 + + + 0 0 + + 0 0 2+
4 r’r 0 + 0 + + + 0 + 0 + + + 0 + 0 + 0 + + 0 + + 0 0 2+
5 rr 0 0 0 + + + 0 + + + + + 0 + 0 + 0 + 0 0 + 0 0 0 2+
6 rr 0 0 0 + + + 0 + + + + + + 0 0 + 0 + + 0 + + 0 0 2+
7 rr 0 0 0 + + + 0 + 0 + 0 0 0 + 0 + + 0 + + 0 + 0 0 2+
8 rr 0 0 0 + + + 0 + 0 + + + 0 0 0 + 0 + 0 + 0 + 0 0 2+
9 rr 0 0 0 + + + 0 + + + + 0 + 0 0 + 0 + + + + 0 0 0 2+
10 R1R1 + + 0 0 + 0 + 0 + + 0 + 0 + 0 + 0 + 0 + + 0 0 0 2+
Auto 0 0 0

What type of antibody is suspected from the reactions observed on the panel?

In this case, panel cells were treated with DTT. The sample was retested. All reactions with the DTT-treated panel cells were negative following testing with LISS at AHG. What does this suggest about the specificity of this antibody?

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EP, a 26-year-old Caucasian female student, tested her own blood during a student lab on antibody id...

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